Jenway 6850 Bedienungsanleitung

Operating Manual REV A/06-12 6850 PRISM PC Software

10Status font Setup font of status bar Customize Display Setup Peaks Identify Spectrum Peaks Valleys Identify Spectrum Valleys Zoom Activate

11Set Unit Select concentration unit Turn on/off W lamp Turn on/off Tungsten lamp Turn on/off D2 lamp Turn on/off Deuterium lamp D2/W Switch

12Divide Divide one spectrum from another Savitzky-Golay Smoothing Filter Smooth a spectrum with the Savitzky-Golay method filter Derivate Displ

13SECTION 4 – PHOTOMETRIC MEASUREMENT 4.1 WAVELENGTH SELECTION A single wavelength photometric measurement is made by firstly selecting the

14SECTION 5 – MULTIWAVELENGTH / QUANTITATION The multi wavelength and quantitation measurement mode enables measurements of absorbance, % transmitt

155.3 SAMPLE INFORMATION The Information tab allows the user to enter sample and standard information. Additional notes can be entered into the Me

165.4.4 Control Window – Recalculate Select the Recalculate button to re-evaluate the concentration result when the Quantitation mode is enabled.

175.5.1.2 Entering Known Calibration Curve Constants The Use Standard Samples checkbox should be clear and the Curve Fit option should be set to

18 Once the standard measurement is complete and the name and concentration information has been entered, select OK to transfer the information to

195.5.3 Sample Measurement To quantify an unknown sample select the Sample tab and select the Start button. A new window will open allowing the

2Contents SECTION 1 – Introduction ... 41.1 PC SOFTWARE DESCR

20SECTION 6 – SPECTRUM The spectrum measurement mode enables measurements of absorbance or % transmittance over a range of wavelengths to be perfor

216.4 SAMPLE MEASUREMENTS Insert a cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette

226.5.3 Spectrum Zoom Function Select the Zoom function icon from the toolbar. Position the cursor in the upper-left corner of the area you want

236.5.7 Remeasure (Re-plot) Spectrum Click on the toolbar. A dialogue box appears asking the user to specify the frequency of the data points i

246.6.2 Delete Displayed Spectrum Select the spectrum to be deleted in the active spectrum drop down box (see 6.6). Click on the toolbar to remov

25SECTION 7 – KINETICS The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to be measured over a period

267.4 SAMPLE MEASUREMENTS Insert a cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette

277.5.3 Spectral Points Analysis Select the Scan spectrum icon from the toolbar. Move the cursor over the kinetics spectrum to trace the s

287.6.1 Kinetics Display and Print Options The display and print options for the selected scan can be accessed by selecting the icon on the too

29SECTION 8 – DNA/PROTEIN The DNA/Protein measurement mode allows the user to measure multi-wavelength absorbance ratios, such as 260nm/280nm and

36.5.4 Spectral Points Analysis ... 226.5.5 S

308.3 DETERMINATION OF NUCLEIC ACID CONCENTRATION Select the required method parameters as in 8.2. 8.3.1 Sample Measurements To quantify an unkno

318.4.2 Constructing a New Calibration Curve 8.4.2.1 Measuring Standard Samples Select the Standard tab. Insert the first standard into the sa

32 A new window will open allowing the sample name to be edited. The actual photometric reading of the standard will be displayed in the Readout wi

33SECTION 9 – APPENDIX The multicell accessory measurement mode allows the user to measure a sample’s photometric absorbance or %transmittance at

34SECTION 10 – TECHNICAL SUPPORT 10.1 TECHNICAL SUPPORT Jenway have a dedicated Technical Support team made up of experienced scientists who are on

4SECTION 1 – Introduction 1.1 PC SOFTWARE DESCRIPTION The Prism PC software allows the user to fully control the functionality of the Jenway 6850

53. Select Next. 4. Enter the requested user information and select Next. 5. Choose install path, then select Next. 6. Choose a program fold

67. Click Finish to finish the installation. 8. Connect the PC and Spectrophotometer with the USB Cable. 2.3 INITIALISATION The Prism PC softwar

72.4 SETUP COMMUNICATION PORT Select the Spectrophotometer menu and open the Comm Port Setup option. In the Communication Hub Setup window se

8SECTION 3 – PRISM SOFTWARE INTERFACE 3.1 MAIN WINDOW The Main Window is made up of four main areas. 1. Menu Bar 2. Toolbar 3. Data Window 4. Statu

93.1.1 Menu Bar and Toolbar Options Menu Bar Option Sub Menu Option Toolbar Icon Function File New New Multi Wavelength Measurement New Spectru