Jenway Genova Nano German Bedienungsanleitung

Genova PlusSpectrophotometerOperating Manual736 505 REV A/11-12

SECTION 1 - Introduction INSTRUMENT DESCRIPTION1.1 The Genova Plus is a UV/visible spectrophotometer dedicated to life science analysis. This spec

To perform a measurement place the sipper tubing into the sample and press the key below the sipper pump icon. Confirmation will be needed to start the

The settings menu is the same as the sipper pump settings except for the Peltier icon in the top left hand corner. Pressing the key adjacent

SECTION 19 – Maintenance and service19.1 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The sample ar

SECTION 20 – Troubleshooting20.1 ERROR CODESIf an error code is displayed it will be accompanied by a spanner icon and a symbol to indicate if the

Err 4 Microswitch Failure (service only) This error indicates that the microswitch was not found. The most likely causes of this error are:

20.2 TOUBLESHOOTING GUIDE Issue SolutionUnable to achieve zero absorbance or 100% transmittance when calibratingUnable to achieve a reading when mea

SECTION 21 – Declaration of Conformity104

SECTION 22 – Glossary of iconsMode ICON DescriptionCommon Back key Common Tick icon - Done/yesCommon Cross icon – Cancel/noCommon Prin

Common Eraser icon - Delete letter/numberCommon AB icon - Change letter case/numberCommon DeleteCommon Method icon – opens method selection

Main Menu 12.00 Time/date icon Time & Date Clock icon - Set timeTime & Date Calendar icon - Set dateTime & Date Toggle icon – Swit

Curve fit algorithms Quadratic, quadratic through zero, linear, linear through zero, interpolate Multi-wavelength Range 0 to 9999 Data poin

Concentration Calibrate to zero absorbance or standard Concentration Factor menuConcentration Standard menuConcentration 001 Resets valu

Quantitation Number of standardsQuantitation View standard curveQuantitation Curve fit – enables the curve fit to be adjusted Quantitation S

Kinetics Double greater than icon - Increase the time by 5 seconds intervals Kinetics Single greater than icon - Increase the time by 1 se

Accessories No single cell - Method created on a unit with a single cell accessory fittedAccessories 8 cell turret icon - the automatic 8 cel

UKBibby Scientific Ltd.Beacon Road, Stone Staffordshire ST15 0SAUnited KingdomTel: +44 (0)1785 812121 Fax: +44 (0)1785 810405 e-mail: sales@bi

SECTION 2 – Installation2.1 UNPACKINGRemove the Genova Plus from the packaging and ensure the following items are included:1. Model Genova Plus

The instrument will initially check for firmware updates (Section 20.3) and then perform several power-on tests before displaying the main menu: Fig 2.

Life Science/Spectrophotometer menu options1. Purity/Spectrum measurement mode2. Concentration plus/Concentration measurement mode3. Back key4. Time a

2.5 REAR PANELThe image below shows the rear panel on the instrument: Fig. 2.5.1 – Rear Panel1. Lamp access panel Allows access to lamp when repl

SECTION 3 – Theory and Practice of Spectroscopy Measurements3.1 THEORY OF SPECTROSCOPY MEASUREMENTUV-visible spectroscopy is the measure

Nucleic acid concentration can also be estimated with the following calculations: Conc (µg/ml) = (Abs@260nm x 62.9) - (Abs@280nm x 36.0) Conc (µg/ml

3.4 GOOD PRACTICE GUIDELINES1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambi

9. Deviations from the Beer-Lambert Law may occur at high and low concentrations giving non-linear response during sample concentration measurements.

SECTION 4 – Instrument Setup4.1 NAVIGATING AND SCREEN SETUP The main life science menu screen is displayed below.Fig 4.1.1 – Life Science Home S

To navigate around the spectrophotometer screen press the soft keys adjacent to icons displayed on the screen. There is a back key which returns to th

To change between the two formats press the key below the toggle icon. Once the current time and date have been set press the key adjacent to the tick

methods can still be opened, deleted and saved but the method parameters cannot be changed. To enter the security code, use the keys at the bottom of

4.6 GLP SETTINGSIn addition to the time and date settings this instrument also has a user ID function. This function enables an individual three d

SPECTROPHOTOMETER MENU OPTIONSSECTION 5 – PhotometricsThe photometrics measurement mode enables simple measurements of absorbance and % transmittance

5.3 CALIBRATIONThe calibration must be performed at the same wavelength at which the sample will be measured. Insert a cuvette contain

SECTION 6 – ConcentrationThe concentration measurement mode enables simple measurements of absorbance and concentration to be performed. In th

This will open a number entry screen. Use the keys at the bottom of the screen to select the digit to be adjusted. Use the keys adjacent to t

6.2.2.3 Using a Standard The standard menu enables the value of a standard to be entered. This function is accessed by pressing the key adja

1 SafetyPlease read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is design

6.3.1 Calibrating to a StandardInsert a cuvette containing the blank solution into the sample chamber and close the instrument lid. Press the key b

6.4.2 Measuring a Sample After Calibrating to a FactorRemove the cuvette containing the blank solution and place a cuvette containing the samp

SECTION 7 – SpectrumThe spectrum measurement mode enables measurements of absorbance or % transmittance over a range of wavelengths to be perfo

7.2.1 Scan SettingsThis function enables the graph y-axis, absorbance or % transmittance operating mode, start and end wavelengths and scan interv

the start wavelength will automatically be set to be one times the scan interval. For example, if the end wavelength is entered as 500nm but the start

7.3 CALIBRATIONIn the spectrum measurement mode the calibration is a baseline scan which is performed across the selected wavelength

Depending on how many data points have been measured either a partial scan and all the post measurement tools will be displayed,

In the peaks and valleys table screen it is possible to display both peaks and valleys, just peaks or just valleys. To display the peaks only press th

or decrease the number. Once the wavelength has been entered press the key adjacent to the tick icon to save and return to the spectral points analysi

SECTION 8 – QuantitationThe quantitation measurement mode enables sample concentrations to be calculated using a standard curve. In this mode a

2 4. Es ist jederzeit auf die sicherheitsrelevanten Daten sämtlicher verwendeter Chemikalien Bezug zu nehmen. Allgemein anerkannte Laborm

8.2.1 Quantitation SettingsThis function enables the absorbance or % transmittance operating mode, wavelength, units, resolution, number of replicate

8.2.1.5 Selecting the Number of Replicate Standard MeasurementsThe number of replicate measurements made for each standard that is used to construc

This opens a number entry screen; use the keys at the bottom of the screen to select the digit to be changed, pressing the key twice to

Remove the cuvette containing the blank solution from the sample chamber and insert the cuvette containing the first standardised solutio

Repeat presses of the key cycles the curve fit between 1. Linear Regression Concentration = Abs x A + B2. Linear Regression Through Zero Concentrat

8.5 DATA ANALYSISIn quantitation the data analysis examines the statistics of the standard curve and algorithm of the curve fit. The curve statistic

SECTION 9 – KineticsThe kinetics measurement mode enables the absorbance or % transmittance of an active molecule to be measured over a period of t

9.2.1 Kinetics SettingsThe settings menu enables the wavelength, units, resolution, graph y-axis, operating mode, standard, factor, measurement tim

To change the maximum absorbance or % transmittance value press the key adjacent to the maximum y-axis value and this opens a number entry scree

9.2.1.5 Selecting Concentration UnitsThe concentration units can be selected from a number of options: no units, %, ppm, EBC, SRM, mEq/l, mEq, M, mM

Contents PageSafety 1SECTION 1 - Introduction 81.1 INSTRUMENT DESCRIPTION 81.2 INSTRUMENT SPECIFICATION 8SECTION 2 – Installation 10

9.2.1.8 Selecting a WavelengthTo adjust the wavelength press the key adjacent to the wavelength icon. This will open the number entry screen

If a start on level has been set the Abs/%T icon is displayed in the centre of the screen. The instrument will take photometric readings un

specifically. This function can be accessed from the statistics menu or from the operating menu by pressing the key adjacent to the kine

SECTION 10 – Multi-wavelengthThe multi-wavelength measurement mode allows the user to measure a sample’s photometric absorbance or %transmittance

10.2.1 Multi-Wavelength SettingsThe settings menu enables the wavelengths, number of wavelengths, units, resolution, concentration calculation equa

10.2.1.5 Setting the Concentration Calculation Equation and FactorsIn the settings menu press the key adjacent to the concentration calculat

LIFE SCIENCE MENU OPTIONSSECTION 11 – Concentration PlusThe concentration plus measurement mode enables simple measurements of absorbance, %transmitta

11.2.2 Concentration Plus SettingsThe concentration plus settings menu enables the wavelength, units, resolution, standard, factor and dilution fac

In the settings menu press the key below the units icon. This opens the unit selection screen which displays all the different units. Use the keys adj

11.2.2.6 Setting the Dilution FactorIn the settings menu press the key adjacent the dilution icon. This screen allows the user to input the relat

6.4 SAMPLE MEASUREMENT 286.4.1 Measuring a Sample After Calibrating to a Standard 286.4.2 Measuring a Sample After Calibrating to a Factor 29SE

Insert a cuvette containing the standard concentration sample solution into the sample chamber and close the instrument lid.Press the key below the

11.4.2 Measuring a Sample After Calibrating to a FactorRemove the cuvette containing the blank solution and place a cuvette containing the sampl

SECTION 12 – Purity ScanThe purity scan measurement mode enables measurements of absorbance or % transmittance over a range of wavele

SECTION 13 – Multi-wavelength plusThe multi-wavelength plus measurement mode allows the user to determine the concentration of a sample usi

13.2.1 Multi-wavelength Plus SettingsThe settings menu enables the wavelengths, number of wavelengths, units, resolution, dilution factor, concentr

13.2.1.4 Setting the Dilution FactorIn the settings menu press the key below the dilution icon. This screen allows the user to input the rela

When a reference wavelength is included these equations are modified as follows:1. ∑ = xF1*(l1-l4)2. ∑ = (xF1*(l1-l4)) - (xF2*l2)Repeat presses will cy

SECTION 14 – DNAThe DNA measurement mode allows the user to select a method from a list of common nucleic acid measurement tests, including single

The ssDNA settings menu allows the user to adjust measurement specific parameters such as the dilution factor and resolution. Other metho

14.6 260 / 280The 260/280 operating menu enables the purity and concentration of DNA to be estimated according to the following equat

SECTION 10 – MULTI-WAVELENGTH 5110.1 MODE SPECIFIC PARAMETERS 5110.2 METHOD SET UP 5110.2.1 Multi-Wavelength Settings 5210.2.1.1 Setting the N

The Variable Ratio settings menu allows the user to adjust measurement specific parameters such as the dilution factor and resolution but the

SECTION 15 – ProteinThe protein measurement mode allows the user to select a method from a list of common protein assays, including Pierce 660, BCA

The BCA settings menu allows the user to adjust measurement specific parameters such as the number of standards and result resolution. Other me

15.6 BIURET ASSAY The Biuret operating menu enables the user to perform the Biuret protein assay at the specified wavelength of 540nm.

SECTION 16 – OD 600The OD 600 measurement mode enables optical density measurements of cell cultures and broths to be performed. In this measuremen

The settings menu is accessed through the operating menu by pressing the key adjacent to the settings icon. In the settings menu press the key bel

9.999e+19 can be entered. The standard value can be reset to 1.000e+00 by pressing the key adjacent to the 001 icon. Once the standard value has been

16.2.2.4 Setting the Dilution FactorIn the settings menu press the key adjacent the dilution icon. This screen allows the user to input the relat

16.3.1 Calibrating to a StandardInsert a cuvette containing the blank solution into the sample chamber and close the instrument lid. Press the key

16.4.2 Measuring a Sample After Calibrating to a FactorRemove the cuvette containing the blank solution and place a cuvette containing the samp

SECTION 15 – PROTEIN 6915.1 PROTEIN MENU OPTIONS 6915.2 PIERCE 660 ASSAY 6915.3 BCA ASSAY 6915.4 BRADFORD ASSAY 7015.5 LOWRY ASSAY 7015.6

SECTION 17 – Saving, printing and autologgingThe utility toolbar in the operating menu provides access to printing, print setup options, opening, s

The default method name will be displayed. To save the method under the default name press the key adjacent to the tick icon. To change the name o

17.2 OPENING METHODSMethods can be opened from the internal memory of the instrument or from a USB memory stick. Before a method can be open

17.3 DELETING METHODSIn the operating menu press the key adjacent to the method icon to open the method selection menu and use the keys adja

The saved results are listed alphabetically with the date and time that the result was generated. If there is already a result saved under t

17.6 DELETING RESULTSResults can only be deleted if a valid USB memory stick is inserted into the front of the instrument.In the operating menu press

Once the required printout destination and language has been selected press the key adjacent to the tick icon to save and return to the operating menu

icon. Repeat presses of the key will cycle between a tick and a cross icon for selected or deselected. To select the data interval press the key adjac

Once the number of repetitions is increased above zero, if the results are autologged to a USB memory stick, the batch ABC icon will be displayed. To

the results that are going to be recorded. Press the key adjacent to the tick icon to confirm selection. Press the key adjacent to the cross icon to ca

718.2.3.1 Automatic 8 cell turret 9018.2.3.2 Peltier 9018.2.3.3 Sipper pump 9118.2.3.4 Combined sipper Peltier pump 9318.3 USING THE ACCESSOR

SECTION 18 – Accessories and spare parts18.1 OPTIONAL ACCESSORIESPart Code Description of Accessory660 101 Internal printer735 401 Automatic

Squeeze the grey plastic clips together so that the printer top opens. Slot the printer into the top of the instrument and push down until it fits flush

This will expose the bottom of the sample chamber with the power supply connection needed to operate the active accessories. 18.2.3.1 Automatic 8 cel

When the accessory is fitted the instrument will look like this.18.2.3.3 Sipper pumpFor this accessory as well as removing the passive accessory base

For sipping:1. Connect the sipper pump tubing to the outlet port on the flow-through cuvette.2. Secure the tubing using the clip on the righthand side

6. Connect a suitable length of sipper pump tubing to the external outlet port.7. Insert one end of the capillary tube into the sipper pump tubing, as

Press the key below the 8 cell turret icon to highlight the icon and the two arrow icons above. Press the keys adjacent to the arrow icons to increase

This opens the Peltier settings screen. Use the keys at the bottom of the screen to select the digit to be changed and use the arrow icons to increase

Confirmation will be needed to start the sipper pump. Press the key adjacent to the tick icon to confirm and start the sipper pump. Press the ke

To fine tune the amount of sample uptake press the key below the plus or minus icon to increase or decrease the amount of sample taken up. The re